Pulsed and parallel-polarization EPR characterization of the photosystem II oxygen-evolving complex

被引：101

作者：

Britt, RD ^{[1
]}

Peloquin, JM ^{[1
]}

Campbell, KA ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2000年 / 29卷

关键词：

manganese cluster; photoactivation; oxygen evolution; ENDOR;

D O I：

10.1146/annurev.biophys.29.1.463

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photosystem II uses visible light to drive the oxidation of water, resulting in bioactivated electrons and protons, with the production of molecular oxygen as a byproduct. This water-splitting reaction is carried out by a manganese cluster/tyrosine radical ensemble, the oxygen-evolving complex. Although conventional continuous-wave, perpendicular-polarization electron paramagnetic resonance (EPR) spectroscopy has significantly advanced our knowledge of the structure and function of the oxygen-evolving complex, significant additional information can be obtained with the application of additional EPR methodologies. Specifically, parallel-polarization EPR spectroscopy can be used to obtain highly resolved EPR spectra of integer spin Mn species, and pulsed EPR spectroscopy with electron spin echo-based sequences, such as electron spin echo envelope modulation and electron spin echo-electron nuclear double resonance, can be used to measure weak interactions obscured in continuous-wave spectroscopy by inhomogeneous broadening.

引用

页码：463 / 495

页数：33

共 98 条

[31] INTERMEDIATES OF A POLYNUCLEAR MANGANESE CENTER INVOLVED IN PHOTOSYNTHETIC OXIDATION OF WATER [J].