The ribosomal peptidyl transferase

被引：121

作者：

Beringer, Malte ^{[1
]}

Rodnina, Marina V. ^{[1
]}

机构：

[1] Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

来源：

MOLECULAR CELL | 2007年 / 26卷 / 03期

关键词：

PEPTIDYLTRANSFERASE ACTIVE-SITE; SUBSTRATE-ASSISTED CATALYSIS; AMINOACYL-TRANSFER-RNA; BOND FORMATION; CRYSTAL-STRUCTURE; BASE-PAIR; STRUCTURAL INSIGHTS; PUROMYCIN REACTION; MECHANISM; INTERMEDIATE;

D O I：

10.1016/j.molcel.2007.03.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide bond formation on the ribosome takes place in an active site composed of RNA. Recent progress of structural, biochemical, and computational approaches has provided a fairly detailed picture of the catalytic mechanism of the reaction. The ribosome accelerates peptide bond formation by lowering the activation entropy of the reaction due to positioning the two substrates, ordering water in the active site, and providing an electrostatic network that stabilizes the reaction intermediates. Proton transfer during the reaction appears to be promoted by a concerted proton shuttle mechanism that involves ribose hydroxyl groups on the tRNA substrate.

引用

收藏

页码：311 / 321

页数：11

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