λN-GFP:: An RNA reporter system for live-cell imaging

被引：180

作者：

Daigle, Nathalie ^{[1
]}

Ellenberg, Jan ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Unit, D-69117 Heidelberg, Germany

来源：

NATURE METHODS | 2007年 / 4卷 / 08期

关键词：

D O I：

10.1038/NMETH1065

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a GFP- based RNA reporter system ( gimel(N)- GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine- rich peptide derived from the phage gimel N protein, gimel(N22), which binds a unique minimal RNA motif and can be used to tag any RNA molecule. gimel(N)- GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.

引用

页码：633 / 636

页数：4

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