Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms

被引：48

作者：

Chemler, Joseph A. ^{[1
]}

Yan, Yajun ^{[1
]}

Leonard, Effendi ^{[1
]}

Koffas, Mattheos A. G. ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Chem & Biol Engn, Buffalo, NY 14260 USA

来源：

ORGANIC LETTERS | 2007年 / 9卷 / 10期

关键词：

CELL-SUSPENSION CULTURES; ESCHERICHIA-COLI; SUBSTRATE-SPECIFICITY; ENZYMATIC FORMATION; ALPHA-GLUCOSIDASE; CHALCONE SYNTHASE; NATURAL-PRODUCTS; GENE-CLUSTER; COA LIGASE; BIOSYNTHESIS;

D O I：

10.1021/ol0703736

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Flavonoids are plant secondary metabolites often used as nutraceutical supplements, but a growing number of unnatural flavonoids are being investigated as therapeutic agents. Cultures of Saccharomyces cerevisiae expressing recombinant flavonoid enzymes, including 4-coumaroyl:CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), and flavanone 3 beta-hydroxylase (FHT), produced novel flavanones and dihydroflavonols when fed with a number of aromatic acrylic acids. The flavonoid network also exhibited broad substrate specificity by converting muconic acid into a unique polypropanoid.

引用

页码：1855 / 1858

页数：4

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