Dielectric relaxation measurements of 12 kbp plasmid DNA

被引：38

作者：

Bakewell, DJ

Ermolina, I

Morgan, H ^{[1
]}

Milner, J

Feldman, Y

机构：

[1] Univ Glasgow, Dept Elect & Elect Engn, Bioelect Res Ctr, Glasgow G12 8LT, Lanark, Scotland

[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[3] Hebrew Univ Jerusalem, Dept Appl Phys, IL-91904 Jerusalem, Israel

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1493卷 / 1-2期

关键词：

DNA polarisability; dielectric spectroscopy; counterion fluctuation;

D O I：

10.1016/S0167-4781(00)00176-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dielectric properties of 12 kbp plasmid DNA have been measured as a function of temperature in the range 5 degrees C to 40 degrees C. Time domain reflectometry was used to obtain dielectric data over the frequency range from 200 kHz to 3 GHz. Values of the frequency dependent polarisability per DNA macromolecule have been determined from the measurements. Possible mechanisms that could account for the dielectric dispersion are also discussed, in particular the counterion fluctuation model of Manning-Mandel-Oosawa. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 158

页数：8

共 45 条

[1] POLY-ELECTROLYTE THEORIES AND THEIR APPLICATIONS TO DNA [J].