Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan

被引:59
作者
Gotanda, Yuhko [1 ]
Iwata, Akiko [1 ]
Ohnuma, Hitoshi [1 ]
Yoshikawa, Akira [1 ]
Mizoguchi, Hideaki [1 ]
Endo, Kazunori [1 ]
Takahashi, Masaharu [1 ]
Okamoto, Hiroaki [1 ]
机构
[1] Jichi Med Univ, Sch Med, Dept Infect & Immun, Div Virol, Minamimaki, Tochigi 3290498, Japan
关键词
hepatitis E virus; subclinical infection; PCR; genotype; phylogenetic analysis; GENOME NUCLEOTIDE-SEQUENCE; GENETIC-HETEROGENEITY; FULMINANT-HEPATITIS; SPORADIC ACUTE; WILD BOAR; HEV; ANTIBODIES; IDENTIFICATION; TRANSMISSION; INDIVIDUALS;
D O I
10.1002/jmv.20834
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ongoing subclinical infection of hepatitis E virus (HEV) has not been fully studied. In the present study, serum samples were collected from 6700 voluntary blood donors with an elevated alanine aminotransferase (ALT) level of 61-476 IU/I at a Japanese Red Cross Blood Center, and were tested for the presence of IgG, IgM and IgA classes of antibodies to HEV (anti-HEV) by inhouse ELISA and HEV RNA by nested RT-PCR. Overall, 479 blood donors (7.1%) were positive for anti-HEV IgG, including 8 donors with anti-HEV IgM and 7 donors with anti-HEV IgA. Among the nine donors with anti-HEV IgM and/or anti-HEV IgA, six had detectable HEV RNA. The presence of HEV RNA was further tested in 10-sample minipools of sera from the remaining 6691 donors, and three donors including one without anti-HEV IgG were found to be positive for HEV RNA. When stratified by ALT level, the prevalence of HEV RNA was significantly higher among the 109 donors with ALT >= 201 IU/I than among the 6591 donors with ALT of 61-200 IU/I (2.8% vs. 0.1%, P < 0.0001). The HEV isolates obtained from the nine viremic donors segregated into genotype 3, shared a wide range of identities of 85.6-98.5% and were 87.3-93.9% similar to the Japan-indigenous HEV strain (JRA1), in the 412-nucleoticle sequence of open reading frame 2. This study suggests that approximately 3% of Japanese individuals with ALT >= 201 IU/I have ongoing subclinical infection with various HEV strains. J. Med. Virol. 79: 734-742, 2007. (C) 2007 Wiley-Liss, Inc.
引用
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页码:734 / 742
页数:9
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