Comparison of one-tube multiplex PCR, automated ribotyping and intergenic spacer (ITS) sequencing for rapid identification of Acinetobacter baumannii

被引:137
作者
Chen, T.-L.
Siu, L.-K.
Wu, R. C.-C.
Shaio, M.-F.
Huang, L.-Y.
Fung, C.-P.
Lee, C.-M.
Cho, W.-L. [1 ]
机构
[1] Natl Yang Ming Univ, Inst Trop Med, Sch Med, Taipei 112, Taiwan
[2] Taipei Vet Gen Hosp, Sect Gen Med, Taichung, Taiwan
[3] Taipei Vet Gen Hosp, Infect Dis Sect, Dept Med, Taichung, Taiwan
[4] Natl Yang Ming Univ, Inst Trop Med, Sch Med, Taichung, Taiwan
[5] Natl Hlth Res Inst, Div Clin Res, Taichung, Taiwan
[6] Hung Kuang Univ, Dept Med Res, Kuang Tien Gen Hosp, Inst Clin Nutr, Taichung, Taiwan
[7] Mackay Mem Hosp, Div Infect Dis, Taipei, Taiwan
[8] Mackay Mem Hosp, Nursing & Management Coll, Taipei, Taiwan
关键词
Acinetobacter baumannii; automated ribotyping; identification; intergenic spacer region; multiplex PCR; sequencing;
D O I
10.1111/j.1469-0691.2007.01744.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Acinetobacter baumannii has emerged as a serious cause of nosocomial infections. Rapid identification of this pathogen is required so that appropriate therapy can be given and outbreaks controlled. This study evaluated a multiplex PCR and an automated ribotyping system for the rapid identification of Acinetobacter baumannii. In total, 22 different reference strains and 138 clinical isolates of Acinetobacter spp., identified by 16S-23S rRNA intergenic spacer (ITS) sequence analysis, were evaluated. All A. baumannii isolates (82 clinical isolates and one reference strain) were identified by the multiplex PCR method (specificity 100%). The sensitivity and specificity of the ribotyping system for identification of A. baumannii were 85.5% (71/83) and 93.5% (72/77), respectively. An additional 100 clinical isolates belonging to the Acinetobacter calcoaceticus-A. baumannii complex were used to compare these two methods for identification of A. baumannii, and this comparison revealed a level of disagreement of 14% (14 isolates). The accuracy of the multiplex PCR was 100%, which was confirmed by sequence analysis of the ITS and recA gene of these isolates. Thus, the multiplex PCR method dramatically increased the efficiency and speed of A. baumannii identification.
引用
收藏
页码:801 / 806
页数:6
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