Effects of combined expression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection

被引：52

作者：

Bieri, S ^{[1
]}

Potrykus, I ^{[1
]}

Fütterer, J ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Plant Sci, CH-8092 Zurich, Switzerland

来源：

MOLECULAR BREEDING | 2003年 / 11卷 / 01期

关键词：

beta-1,3-glucanase; anti-fungal protein; chitinase; fungal tolerance; ribosome inactivating protein; synergistic effect; transgenic wheat; FUNGAL RESISTANCE; TOBACCO; CHITINASES; PLANTS; TRANSFORMATION; PROMOTER; GENES; SPECIFICITY; RIBOSOMES; VIRUSES;

D O I：

10.1023/A:1022145915983

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Transgenic wheat plants (variety Frisal) constitutively expressing a number of potential antifungal proteins alone or in combinations were generated and tested for increased resistance to Blumeria graminis f. sp. tritici (powdery mildew) in a detached leaf infection assay. The most significative rate of protection was obtained with an apoplastic ribosome-inactivation protein from barley seed. Apoplastic Barnase was less efficient and individual plant lines harbouring a barley seed chitinase and beta-1,3-glucanase showed line specific phenotypes from increased resistance to increased susceptibility. Combination by crossing of three barley seed proteins did not lead to significant improvement of protection.

引用

页码：37 / 48

页数：12

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