In vitro fluconazole susceptibility of 1565 clinical isolates of Candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines

被引：11

作者：

Testore, GP

Dori, L

Buonomini, AR

Schito, GC

Soro, O

Fortina, G

Andreoni, S

Carlone, N

Tullio, V

Andreoni, M ^{[1
]}

机构：

[1] Univ Roma Tor Vergata, Dept Publ Hlth, Rome, Italy

[2] Univ Genoa, Div Microbiol, Surg Anaestesiol & Organ Transplantat Sci Dept, Genoa, Italy

[3] AO Osped Maggiore Car, Microbiol & Virol Div, Novara, Italy

[4] Univ Turin, Dept Publ Hlth & Microbiol, Turin, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2004年 / 50卷 / 03期

关键词：

D O I：

10.1016/j.diagmicrobio.2004.07.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We determined the in vitro activity of fluconazole against 1565 clinical Candida spp. isolates collected from different specimens of non-AIDS outpatients and inpatients in 3 different regions of Italy. Susceptibility testing was performed by agar disk diffusion using the NCCLS document M44-A guidelines. Candida albicans was the most frequently isolated yeast (68%) followed by C. glabrata (15%), C tropicalis (5%), C. parapsilosis (5%), and C. krusei (5%). Other yeasts represented 4% of all isolates. Of the 1565 isolates tested, 1449 (92.6%) were susceptible (S) to fluconazole, 43 (2.7%) were susceptible dose-dependent (S-DD) and 73 (4.7%) were resistant (R). Almost all (98.2%) of the C. albicans isolates were classified as S or S-DD. Despite its widespread use, fluconazole displayed good activity against the isolates we tested, and the disk diffusion method was confirmed as a reliable approach to the evaluation of in vitro susceptibility of yeasts to this antimycotic agent. (C) 2004 Elsevier Inc. All rights reserved.

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页码：187 / 192

页数：6

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