The susceptibility of 3 laboratory strains of Aedes albopictus (Skuse) (Sanhsia [SH], Yungho [YH], Liyang [LY]), and 1 strain of Culex tritaeniorhynchus Giles from northern and central Taiwan were compared for susceptibility to the MQ1-2 strain of Japanese encephalitis (JE) virus. The median infective dose (MID50) by intrathoracic inoculation was 0.23, 0.76, 1.60, and -0.03 log(10) WMICLD50 (50% weanling mice intracranial lethal dose) with Ae. albopictus SH, YH, LY, and Cx. tritaeniorhynchus, respectively. After feeding on a sweetened blood-virus mixture, the oral MID50 was 2.03, 4.32, and 4.98 log(10) WMICLD50 for SH, YH, and LY, respectively,, and 1.02 log(10) WMICLD50 for Cx. tritaeniorhynchus. The SH Ae. albopictus strain tramitted virus to normal mice after 14 d, with an average transmission rate of 45%. Based on these results, the SH strain was the most susceptible and important potential vector among 3 Ae. albopictus strains for the sympatric MQ1-2 strain of JE.