cDNA cloning of biologically active chicken interleukin-18

被引：96

作者：

Schneider, K

Puehler, F

Baeuerle, D

Elvers, S

Staeheli, P

Kaspers, B

Weining, KC

机构：

[1] Univ Freiburg, Dept Virol, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany

[2] Univ Munich, Inst Tierphysiol, D-80539 Munich, Germany

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 2000年 / 20卷 / 10期

关键词：

D O I：

10.1089/10799900050163244

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By searching a chicken EST database, we identified a cDNA clone that appeared to contain the entire open reading frame (ORF) of chicken interleukin-18 (ChIL-18). The encoded protein consists of 198 amino acids and exhibits approximately 30% sequence identity to IL-18 of humans and various others mammals. Sequence comparisons reveals a putative caspase-1 cleavage site at aspartic acid 29 of the primary translation product, indicating that mature ChIL-18 might consist of 169 amino acids. Bacterially expressed ChIL-18 in which the N-terminal 29 amino acids of the putative precursor molecule were replaced by a histidine tag induced the synthesis of interferon-gamma (IFN-gamma) in cultured primary chicken spleen cells, indicating that the recombinant protein is biologically active.

引用

收藏

页码：879 / 883

页数：5

相关论文

共 32 条

[21] Interleukin-18 (IFNγ-inducing factor) induces IL-8 and IL-1β via TNFα production from non-CD14+ human blood mononuclear cells [J].

Puren, AJ ;

Fantuzzi, G ;

Gu, Y ;

Su, MSS ;

Dinarello, CA .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (03) :711-721

[22] Gene expression, synthesis, and secretion of interleukin 18 and interleukin 1β are differentially regulated in human blood mononuclear cells and mouse spleen cells [J].

Puren, AJ ;

Fantuzzi, G ;

Dinarello, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :2256-2261

[23] IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF kappa B [J].

Robinson, D ;

Shibuya, K ;

Mui, A ;

Zonin, F ;

Murphy, E ;

Sana, T ;

Hartley, SB ;

Menon, S ;

Kastelein, R ;

Bazan, F ;

OGarra, A .

IMMUNITY, 1997, 7 (04) :571-581

[24] CHICKEN INTERFERON GENE - CLONING, EXPRESSION, AND ANALYSIS [J].

SEKELLICK, MJ ;

FERRANDINO, AF ;

HOPKINS, DA ;

MARCUS, PI .

JOURNAL OF INTERFERON RESEARCH, 1994, 14 (02) :71-79

[25] A family of genes coding for two serologically distinct chicken interferons [J].

Sick, C ;

Schultz, U ;

Staeheli, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (13) :7635-7639

[26] Ectromelia, vaccinia and cowpox viruses encode secreted interleukin-18-binding proteins [J].

Smith, VP ;

Bryant, NA ;

Alcamí, A .

JOURNAL OF GENERAL VIROLOGY, 2000, 81 :1223-1230

[27] NITRIC-OXIDE - A MACROPHAGE PRODUCT RESPONSIBLE FOR CYTOSTASIS AND RESPIRATORY INHIBITION IN TUMOR TARGET-CELLS [J].

STUEHR, DJ ;

NATHAN, CF .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (05) :1543-1555

[28]

Sundick RS, 1997, J IMMUNOL, V159, P720

[29] Purification and characterization of the human interleukin-18 receptor [J].

Torigoe, K ;

Ushio, S ;

Okura, T ;

Kobayashi, S ;

Taniai, M ;

Kunikata, T ;

Murakami, T ;

Sanou, O ;

Kojima, H ;

Fujii, M ;

Ohta, T ;

Ikeda, M ;

Ikegami, H ;

Kurimoto, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (41) :25737-25742

[30] A chicken homolog of mammalian interleukin-1β:: cDNA cloning and purification of active recombinant protein [J].

Weining, KC ;

Sick, C ;

Kaspers, B ;

Staeheli, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 258 (03) :994-1000

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