Ex vivo drug and irradiation sensitivities in hypermutated and unmutated forms of chronic lymphocytic leukemia cells

被引:7
作者
Kivekäs, I
Tobin, G
Thunberg, U
Vilpo, L
Sundström, C
Rosenquist, R
Vilpo, J [1 ]
机构
[1] Jorvi Hosp, HYKS, Dept Clin Chem, SF-02740 Espoo, Finland
[2] Tampere Univ Hosp, Lab Ctr, Tampere, Finland
[3] Uppsala Univ, Dept Oncol Radiol & Clin Immunol, Uppsala, Sweden
[4] Uppsala Univ, Dept Genet & Pathol, Uppsala, Sweden
[5] Tampere Univ, Sch Med, Tampere, Finland
关键词
CLL; chronic lymphocytic leukemia; immunoglobulin gene; somatic hypermutation; drug sensitivity;
D O I
10.1016/S0145-2126(02)00169-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Several investigators have now established that chronic lymphocytic leukemia (CLL) is not a uniform disease entity, since approximately half of the cases of CLL have undergone immunoglobulin V region (IgV) hypermutation, whereas the other half display unmutated Ig genes. The median survival time of mutated CLL (M-CLL) cases has been shown to be approximately twice as long as that for unmutated CLL (UM-CLL), but no clear explanation for this difference is currently available. In this work, we have investigated a cohort of previously untreated CLL patients, to see whether the ex vivo sensitivities of leukemic cells of 16 UM-CLL patients differ from those of 8 M-CLL patients, using nine different drugs and two types of irradiation. Our results demonstrated very similar ex vivo sensitivities and tumor cell heterogeneity of sensitivity of UM-CLL and M-CLL cells when tested against chlorambucil, 2-chloro-2-deoxyadenosine, cyclosporin A, cis-platinum(II)diammine-dichloride, doxorubicin hydrochloride, 2-fluoroadenine-9-beta-D-arabinofuranoside, prednisolone sodium succinate, verapamil, vincristine, gamma-irradiation, and UV-irradiation. This indicates that de novo chemo/radiosensitivity cannot explain the survival difference observed between UM-CLL and M-CLL. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:337 / 341
页数:5
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