Identification of group B streptococcal Sip protein, which elicits cross-protective immunity

被引:121
作者
Brodeur, BR
Boyer, M
Charlebois, I
Hamel, J
Couture, F
Rioux, CR
Martin, D
机构
[1] CHU Quebec, Unite Rech Vaccinol, Quebec City, PQ G1V 4G2, Canada
[2] Univ Laval, St Foy, PQ G1K 4G2, Canada
关键词
D O I
10.1128/IAI.68.10.5610-5618.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A protein of group B streptococci (GBS), named Sip for surface immunogenic protein, which is distinct from previously described surface proteins, was identified after immunological screening of a genomic library. Immunoblots using a Sip-specific monoclonal antibody indicated that a protein band with an approximate molecular mass of 53 kDa which did not vary in size was present in every GBS strain tested. Representatives of all nine GBS serotypes were included in the panel of strains. Cloning and sequencing of the sip gene revealed an open reading frame of 1,305 nucleotides coding for a polypeptide of 432 amino acid residues, with a calculated pi of 6.84 and molecular mass of 45.5 kDa, Comparison of the nucleotide sequences from six different strains confirmed with 98% identity that the sip gene is highly conserved among GBS isolates. N-terminal amino acid sequencing also indicated the presence of a 25-amino-acid signal peptide which is cleaved in the mature protein. More importantly, immunization with the recombinant Sip protein efficiently protected CD-I mice against deadly challenges with six GBS strains of serotypes Ia/c, Ib, II/R, III, V, and VI. The data presented in this study suggest that this highly conserved protein induces cross-protective immunity against GBS infections and emphasize its potential as a universal vaccine candidate.
引用
收藏
页码:5610 / 5618
页数:9
相关论文
共 47 条
  • [1] Areschoug T, 1999, INFECT IMMUN, V67, P6350
  • [2] IMMUNIZATION OF PREGNANT-WOMEN WITH A POLYSACCHARIDE VACCINE OF GROUP-B STREPTOCOCCUS
    BAKER, CJ
    RENCH, MA
    EDWARDS, MS
    CARPENTER, RJ
    HAYS, BM
    KASPER, DL
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (18) : 1180 - 1185
  • [3] CORRELATION OF MATERNAL ANTIBODY DEFICIENCY WITH SUSCEPTIBILITY TO NEONATAL GROUP-B STREPTOCOCCAL INFECTION
    BAKER, CJ
    KASPER, DL
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1976, 294 (14) : 753 - 756
  • [4] ANTIBODY TO GROUP B-STREPTOCOCCUS TYPE-III IN HUMAN-SERA MEASURED BY A MOUSE PROTECTION TEST
    BALTIMORE, RS
    BAKER, CJ
    KASPER, DL
    [J]. INFECTION AND IMMUNITY, 1981, 32 (01) : 56 - 61
  • [5] The Tat protein export pathway
    Berks, BC
    Sargent, F
    Palmer, T
    [J]. MOLECULAR MICROBIOLOGY, 2000, 35 (02) : 260 - 274
  • [6] BEVANGER L, 1979, ACTA PATH MICRO IM B, V87, P51
  • [7] Invasive group B streptococcal disease: The emergence of serotype V
    Blumberg, HM
    Stephens, DS
    Modansky, M
    Erwin, M
    Elliot, J
    Facklam, RR
    Schuchat, A
    Baughman, W
    Farley, MM
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (02) : 365 - 373
  • [8] PspA, a protection-eliciting pneumococcal protein: Immunogenicity of isolated native PspA in mice
    Briles, DE
    King, JD
    Gray, MA
    McDaniel, LS
    Swiatlo, E
    Benton, KA
    [J]. VACCINE, 1996, 14 (09) : 858 - 867
  • [9] Ferrieri P, 1997, ADV EXP MED BIOL, V418, P635
  • [10] PROTECTION OF MICE AGAINST THE LYME-DISEASE AGENT BY IMMUNIZING WITH RECOMBINANT OSPA
    FIKRIG, E
    BARTHOLD, SW
    KANTOR, FS
    FLAVELL, RA
    [J]. SCIENCE, 1990, 250 (4980) : 553 - 556