Deregulation of sphingolipid metabolism in Alzheimer's disease

Abnormal sphingolipid metabolism has been previously reported in Alzheimer's disease (AD). To extend these findings, several sphingolipids and sphingolipid hydrolases were analyzed in brain samples from AD patients and age-matched normal individuals. We found a pattern of elevated acid sphingomyelinase (ASM) and acid ceramidase (AC) expression in AD, leading to a reduction in sphingomyelin and elevation of ceramide. More sphingosine also was found in the AD brains, although sphingosine-1-phosphate (SIP) levels were reduced. Notably, significant correlations were observed between the brain ASM and SIP levels and the levels of amyloid beta (A beta) peptide and hyperphosphorylated tau protein. Based on these findings, neuronal cell cultures were treated with A beta oligomers, which were found to activate ASM, increase ceramide, and induce apoptosis. Pre-treatment of the neurons with purified, recombinant AC prevented the cells from undergoing A beta-induced apoptosis. We propose that ASM activation is an important pathological event leading to AD, perhaps due to A beta deposition. The downstream consequences of ASM activation are elevated ceramide, activation of ceramidases, and production of sphingosine. The reduced levels of SIP in the AD brain, together with elevated ceramide, likely contribute to the disease pathogenesis. (C) 2008 Elsevier Inc. All rights reserved.