Biogenic Silver for Disinfection of Water Contaminated with Viruses

被引：104

作者：

De Gusseme, Bart ^{[1
]}

Sintubin, Liesje ^{[1
]}

Baert, Leen ^{[2
]}

Thibo, Ellen ^{[1
]}

Hennebel, Tom ^{[1
]}

Vermeulen, Griet ^{[1
]}

Uyttendaele, Mieke ^{[2
]}

Verstraete, Willy ^{[1
]}

Boon, Nico ^{[1
]}

机构：

[1] Univ Ghent, Lab Microbial Ecol & Technol, Dept Biochem & Microbiol Technol, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Food Microbiol & Food Preservat, Dept Food Safety & Food Qual, B-9000 Ghent, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 04期

关键词：

ESCHERICHIA-COLI; MURINE NOROVIRUS; NANOPARTICLES; STABILITY; NITRATE; MODEL;

D O I：

10.1128/AEM.02433-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of enteric viruses in drinking water is a potential health risk. Growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. In this study, Lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic Ag-0. The antiviral action of biogenic Ag-0 was examined in water spiked with an Enterobacter aerogenes-infecting bacteriophage (UZ1). Addition of 5.4 mg liter(-1) biogenic Ag-0 caused a 4.0-log decrease of the phage after 1 h, whereas the use of chemically produced silver nanoparticles (nAg(0)) showed no inactivation within the same time frame. A control experiment with 5.4 mg liter(-1) ionic Ag+ resulted in a similar inactivation after 5 h only. The antiviral properties of biogenic Ag-0 were also demonstrated on the murine norovirus 1 (MNV-1), a model organism for human noroviruses. Biogenic Ag-0 was applied to an electropositive cartridge filter (NanoCeram) to evaluate its capacity for continuous disinfection. Addition of 31.25 mg biogenic Ag-0 m(-2) on the filter (135 mg biogenic Ag-0 kg(-1) filter medium) caused a 3.8-log decline of the virus. In contrast, only a 1.5-log decrease could be obtained with the original filter. This is the first report to demonstrate the antiviral efficacy of extracellular biogenic Ag-0 and its promising opportunities for continuous water disinfection.

引用

页码：1082 / 1087

页数：6

共 44 条

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