The bladder as a bioreactor: Urothelium production and secretion of growth hormone into urine

被引：73

作者：

Kerr, DE

Liang, FX

Bondioli, KR

Zhao, HP

Kreibich, G

Wall, RJ ^{[1
]}

Sun, TT

机构：

[1] USDA ARS, Beltsville Agr Res Ctr, Gene Evaluat & Mapping Lab, Beltsville, MD 20705 USA

[2] NYU, Sch Med, Ronald Perelman Dept Dermatol, Epithelial Biol Unit,Kaplan Canc Ctr, New York, NY 10016 USA

[3] NYU, Sch Med, Dept Pharmacol, Kaplan Canc Ctr, New York, NY 10016 USA

[4] NYU, Sch Med, Dept Urol, Kaplan Canc Ctr, New York, NY 10016 USA

[5] NYU, Sch Med, Dept Cell Biol, Kaplan Canc Ctr, New York, NY 10016 USA

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 01期

关键词：

genetic engineering; transgenic mice; agricultural biotechnology;

D O I：

10.1038/nbt0198-75

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Uroplakin genes are expressed in a bladder-specific and differentiation-dependent fashion. Using a 3.6-kb promoter of mouse uroplakin II gene, we have generated transgenic mice that express human growth hormone (hGH) in their bladder epithelium, resulting in its secretion into the urine at 100-500 ng/ml. The levels of urine hGH concentration remain constant for longer than 8 months. hGH is present as aggregates mostly in the uroplakin-delivering cytoplasmic vesicles that are targeted to fuse with the apical surface. Using the bladder as a bioreactor offers unique advantages, including the utility of all animals throughout their lives. Using urine, which contains little protein and lipid, as a starting material facilitates recombinant protein purification.

引用

页码：75 / 79

页数：5

共 27 条

[1] 2 MAIN GROUPS OF MOUSE MAJOR URINARY PROTEIN GENES, BOTH LARGELY LOCATED ON CHROMOSOME-4 [J].