Eye opening induces a rapid dendritic localization of PSD-95 in central visual neurons

被引:83
作者
Yoshii, A
Sheng, MH
Constantine-Paton, M
机构
[1] MIT, Dept Biol, Cambridge, MA 02139 USA
[2] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA
[3] MIT, Ctr Learning & Memory, Cambridge, MA 02139 USA
[4] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
[5] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA
[6] Massachusetts Gen Hosp, Dept Neurol, Boston, MA 02114 USA
关键词
D O I
10.1073/pnas.0335785100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The membrane-associated guanylate kinase PSD-95 scaffolds N-methyl-(D)-aspartate receptors to cytoplasmic signaling molecules, and associates with other glutamate receptors at central synapses. However, regulation of PSD-95 in vivo is poorly understood. We provide evidence of an activity-dependent redistribution of PSD-95 to dendrites in central visual neurons that is tied to eye opening. Six hours after eye opening, increased dendritic PSD-95 coimmunoprecipitates with the same proportions of stargazin, increased proportions of the N-methyl-(D)-aspartate receptor subunit NR2A, and decreased proportions of NR2B. Sustained high levels of PSD-95 in dendrites are dependent on continued pattern vision in juvenile but not mature animals, suggesting that the stabilization of PSD-95 at synapses may be involved in the control of developmental plasticity.
引用
收藏
页码:1334 / 1339
页数:6
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