Normalization of two-channel microarrays accounting for experimental design and intensity-dependent relationships

被引：19

作者：

Dabney, Alan R.

Storey, John D. ^{[1
]}

机构：

[1] Univ Washington, Dept Biostat, Seattle, WA 98195 USA

[2] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

[3] Texas A&M Univ, Dept Stat, College Stn, TX 77843 USA

来源：

GENOME BIOLOGY | 2007年 / 8卷 / 03期

关键词：

D O I：

10.1186/gb-2007-8-3-r44

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In normalizing two-channel expression arrays, the ANOVA approach explicity incorporates the experimental design in its model, and the MA-plot based approach accounts for intensivity-dependent biases. However, both approaches can lead to inacurate normalization in fairly common scenarios. We propose a method called eCADS for normalizing two-channel microarrays that accounts for both experimental design and intensity-dependent biases. Under reasonable experimental designs, eCADS preserves differential expression relationships and requires only a single array per sample pair.

引用

页数：20

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