Preparation, characterization and application of pyrene-loaded methoxy poly(ethylene glycol)-poly(lactic acid) copolymer nanoparticles

被引:53
作者
Zhang, Y
Zhang, QH
Zha, LS
Yang, WL
Wang, CC
Jiang, XG
Fu, SK [1 ]
机构
[1] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
[2] Minist Educ, Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[3] Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China
[4] Donghua Univ, Coll Mat Sci & Technol, Shanghai 200051, Peoples R China
关键词
biodegradable polymer; pyrene-loaded nanoparticle; methoxy poly(ethylene glycol)-poly(lactic acid); fluorescent marker; tissue distribution;
D O I
10.1007/s00396-004-1068-5
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Pyrene-loaded biodegradable polymer nanoparticles were prepared by incorporating pyrene into the polymer nanoparticles formulated from amphiphilic diblock copolymer, methoxy poly(ethylene glycol)-poly(lactic acid) (MePEG-PLA). Their morphological structure and physical properties were characterized by nuclear magnetic resonance (NMR), dynamic light scattering, fluorescence spectroscopy, transmission electronic microscopy and zeta potential measurements. Further, MePEG-PLA nanoparticles containing pyrene as fluorescent marker were administered intranasally to rats, and the distribution of nanoparticles in the nasal mucosa and the olfactory bulb were visualized by fluorescence microscopy. NMR results confirmed that MePEG-PLA copolymer can form nanoparticles in water, and hydrophilic PEG chains were located on the surface of the nanoparticles. The particle size, zeta potential and pyrene loading efficiency of MePEG-PLA nanoparticles were dependent on the PLA block content in the copolymer. Following nasal administration, the absorption of nanoparticles across the epithelium was rapid, with fluorescence observed in the olfactory bulb at 5 min, and a higher level of fluorescence persisted in the olfactory mucosa than that in the respiratory mucosa. These results show that pyrene could serve as a useful fluorescence probe for incorporation into polymer nanoparticles to study tissue distribution and MePEG-PLA nanoparticles might have a great potential as carriers of hydrophobic drugs.
引用
收藏
页码:1323 / 1328
页数:6
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