Development of normal reference values for seminal reactive oxygen species and their correlation with leukocytes and semen parameters in a fertile population

被引:79
作者
Athayde, Kelly S.
Cocuzza, Marcello
Agarwal, Ashok
Krajcir, Natalie
Lucon, Antonio M.
Srougi, Miguel
Hallak, Jorge
机构
[1] Univ Sao Paulo, Dept Urol, Sao Paulo, Brazil
[2] Ctr Res Reprod, Glickman Urol Inst, Dept Obstet & Gynecol, Cleveland Clin, Cleveland, OH USA
来源
JOURNAL OF ANDROLOGY | 2007年 / 28卷 / 04期
关键词
oxidative stress; male infertility; Endtz test; spermatozoa; standardization;
D O I
10.2164/jandrol.106.001966
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Although reactive oxygen species (ROSs) are clearly implicated in the pathogenesis of male infertility, few studies have attempted to define the basal levels of ROSs in fertile men. Levels of ROSs are highly influenced by the presence of leukocytes and are associated with decreased seminal parameters. The objective of our study was to determine the normal ROS reference values in neat and washed semen of a fertile population and to correlate the leukocyte concentrations with seminal parameters. We evaluated 114 fertile men seeking vasectomy and 47 subfertile patients as a positive control. All samples were subjected to semen analysis and Endtz testing; chemiluminescence assay was used to determine ROS levels. All seminal parameters were significantly higher in the fertile men than in the subfertile patients. In nonleukocytospermic samples, ROS levels were lower in the fertile men than in the subfertile patients in neat (0.29 [0.18, 0.54] vs 0.94 [0.38, 1.51]) (P =.001) and washed semen (5.73 (1.90,14.71] vs 23.4 [9.46,115.55]) (P =.001). Similarly, in samples with leukocytes (Entdz, less than 1 x 10(6)/mL), ROS levels were lower in the fertile men in neat (0.75 [0.27, 1.71] vs 2.0 [0.97, 27.41]) (P =.001) and washed semen (15.85 [4.18, 62.16] vs 239.83 [33.4, 1193.75]) (P <.0001). As expected, samples with leukocytes had significantly higher ROS values in washed and neat semen. In the fertile population, ROSs were positively correlated with leukocytes and negatively correlated with sperm count and motility. In semen samples without leukocytes, the normality cutoff of ROSs was 0.55 x 10(4) counted photons per minute with 76.4% area under the curve (AUC) in the neat samples and 10.0 x 10(4) counted photons per minute with 77% AUC in the washed samples. In semen samples with leukocytes, the cutoff for ROSs in neat samples was 1.25 with 72.7% AUC and 51.5 with 81 % AUC in the washed samples. We defined the cutoff levels of ROSs in a fertile population. Seminal leukocyte levels below 1 x 10(6)/mL were associated with increased ROSs. ROS levels were positively correlated with leukocytes and negatively correlated with sperm motility and concentration. Patients with normal seminal parameters and lower seminal leukocyte levels may benefit from therapeutic interventions that improve semen quality.
引用
收藏
页码:613 / 620
页数:8
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