Phytochelatin synthesis and cadmium localization in wild type of Arabidopsis thaliana

被引:66
作者
Wójcik, M [1 ]
Tukiendorf, A [1 ]
机构
[1] Marie Curie Sklodowska Univ, Dept Plant Physiol, PL-20033 Lublin, Poland
关键词
Arabidopsis thaliana - wild type; cadmium toxicity and tolerance; phytochelatin; X-ray microanalysis;
D O I
10.1007/s10725-004-1592-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although Arabidopsis thaliana is known as a model plant, in molecular studies, as well as heavy metal tolerance of higher plants, there have been no detailed studies of its cadmium accumulation, tolerance and cellular distribution in a wild type of this species. In hydroponic experiments the wild type of A. thaliana (L.) Heynh cv. Columbia plants grew at cadmium concentrations varying from 5 to 100 muM with phytotoxicity symptoms depending on the concentration and time of application. The concentration of cadmium in roots and shoots increased from 0.28 and 0.08 mg g(-1) d.wt at 5 muM Cd treatment after 7 days to 0.82 and 0.85 mg g(-1) d.wt at 100 muM Cd treatment after 14 days, respectively. Most of the cadmium (69-88% of its total pool) was found in shoot. Cd application induced the biosynthesis of phytochelatins (PCs) in root and shoot tissues. Studies with buthionine sulfoximine [BSO, specific inhibitor of glutathione (GSH) synthesis] supported the presence of Cd-phytochelatin complexes and their role in Cd detoxification and tolerance in wild type of A. thaliana. Cellular distribution of cadmium was examined using energy-dispersive X-ray micro-analysis. Particularly interesting was the observation of cadmium localized in the root pericycle.
引用
收藏
页码:71 / 80
页数:10
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