Application of magnetite and silica-magnetite composites to the isolation of genomic DNA

被引：119

作者：

Taylor, JI ^{[1
]}

Hurst, CD ^{[1
]}

Davies, MJ ^{[1
]}

Sachsinger, N ^{[1
]}

Bruce, IJ ^{[1
]}

机构：

[1] Univ Greenwich, Dept Chem & Life Sci, London SE18 6PF, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 890卷 / 01期

关键词：

magnetic sorbents; magnetite sorbents; silica-magnetite sorbents; DNA;

D O I：

10.1016/S0021-9673(00)00107-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Magnetite and silica-magnetite composites were used as adsorbents for the isolation of genomic DNA from maize kernels. Two methods are described for the preparation of silica-magnetite composites, both of which afford higher yields of genomic DNA than when using magnetite alone, or a commerically available kit. DNA isolated using silica-magnetite was suitable for use in further applications such as polymerase chain reaction amplification and enzyme digestion. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：159 / 166

页数：8

共 21 条

[1] Magnetizable solid-phase supports for purification of nucleic acids [J].

Bruce, IJ ;

Davies, MJ ;

Howard, K ;

Smethurst, DE ;

Todd, M .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1996, 48 (02) :147-149

[2] Synthesis and characterization of polypyrrole-magnetite-silica particles [J].

Butterworth, MD ;

Bell, SA ;

Armes, SP ;

Simpson, AW .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 183 (01) :91-99

[3] Improved manufacture and application of an agarose magnetizable solid-phase support [J].

Davies, MJ ;

Smethurst, DE ;

Howard, KM ;

Todd, M ;

Higgins, LM ;

Bruce, IJ .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 68 (1-2) :95-112

[4] Isolation of plasmid DNA using magnetite as a solid-phase adsorbent [J].

Davies, MJ ;

Taylor, JI ;

Sachsinger, N ;

Bruce, IJ .

ANALYTICAL BIOCHEMISTRY, 1998, 262 (01) :92-94

[5]

EHLERS B, 1997, BUNDESGESUNDHEITSBLA, V4, P118, DOI DOI 10.1007/BF03044156

[6]

Hafeli U., 1997, Scientific and Clinical Applications of Magnetic Carriers

[7] PCR detection of genetically modified soya and maize in foodstuffs [J].

Hurst, CD ;

Knight, A ;

Bruce, IJ .

MOLECULAR BREEDING, 1999, 5 (06) :579-586

[8]

Iler R.K., 1979, The Chemistry of Silica

[9] AN EFFICIENT METHOD FOR ISOLATION OF RNA AND DNA FROM PLANTS CONTAINING POLYPHENOLICS [J].

JOHN, ME .

NUCLEIC ACIDS RESEARCH, 1992, 20 (09) :2381-2381

[10] DNA isolation from single or half seeds suitable for random amplified polymorphic DNA analyses [J].

Krishna, TG ;

Jawali, N .

ANALYTICAL BIOCHEMISTRY, 1997, 250 (01) :125-127

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