Application of magnetite and silica-magnetite composites to the isolation of genomic DNA

被引：119

作者：

Taylor, JI ^{[1
]}

Hurst, CD ^{[1
]}

Davies, MJ ^{[1
]}

Sachsinger, N ^{[1
]}

Bruce, IJ ^{[1
]}

机构：

[1] Univ Greenwich, Dept Chem & Life Sci, London SE18 6PF, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 890卷 / 01期

关键词：

magnetic sorbents; magnetite sorbents; silica-magnetite sorbents; DNA;

D O I：

10.1016/S0021-9673(00)00107-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Magnetite and silica-magnetite composites were used as adsorbents for the isolation of genomic DNA from maize kernels. Two methods are described for the preparation of silica-magnetite composites, both of which afford higher yields of genomic DNA than when using magnetite alone, or a commerically available kit. DNA isolated using silica-magnetite was suitable for use in further applications such as polymerase chain reaction amplification and enzyme digestion. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：159 / 166

页数：8

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