Study of chromium speciation in normal and diabetic rats by activable enriched stable isotope technique

被引：15

作者：

Feng, WY ^{[1
]}

Qian, QF

Ding, WJ

Chai, ZF

机构：

[1] Chinese Acad Sci, Inst High Energy Phys, Beijing, Peoples R China

[2] Chinese Acad Sci, Lab Nucl Anal Techniques, Beijing, Peoples R China

来源：

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2000年 / 244卷 / 02期

关键词：

D O I：

10.1023/A:1006758803726

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chromium speciation was investigated in the liver cytosol, serum and urine of normal and diabetic rats after a single intravenous injection of enriched stable isotope Cr-50 tracer solution. Sephadex G-25 gel chromatography combined with instrumental neutron activation analysis was used to isolate and characterize protein-bound chromium in the above materials. The results indicate that Cr is mainly combined with a high-molecular-weight protein either in liver cytosol or serum. A low-molecular-weight, Cr-containing compound (LMWCr) was found in all the observed liver, serum and urine samples of both normal and diabetic rats. Chromium is excreted chiefly as LMWCr in urine.

引用

页码：321 / 325

页数：5

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