Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus

被引：30

作者：

Fan, XM

McLachlin, JR

Weaver, RF ^{[1
]}

机构：

[1] Univ Kansas, Dept Biochem Cell & Mol Biol, Lawrence, KS 66045 USA

[2] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

来源：

VIROLOGY | 1998年 / 240卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1006/viro.1997.8944

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-l in vitro. Northern blotting analysis revealed that pkip is a late gene. Attempts to plaque purify a pkip(-) virus were unsuccessful. (C) 1998 Academic Press.

引用

收藏

页码：175 / 182

页数：8

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