GENOTYPING OF 73 UM-SCC HEAD AND NECK SQUAMOUS CELL CARCINOMA CELL LINES

被引：254

作者：

Brenner, J. Chad ^{[1
,2
]}

Graham, Martin P. ^{[1
]}

Kumar, Bhavna ^{[1
,3
]}

Saunders, Lindsay M. ^{[1
]}

Kupfer, Robbi ^{[1
]}

Lyons, Robert H. ^{[4
]}

Bradford, Carol R. ^{[1
]}

Carey, Thomas E. ^{[1
,2
,5
]}

机构：

[1] Univ Michigan, Dept Otolaryngol Head & Neck Surg, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Pathol, Program Cellular & Mol Biol, Ann Arbor, MI 48109 USA

[3] Ohio State Univ, Dept Otolaryngol Head & Neck Surg, Columbus, OH 43210 USA

[4] Univ Michigan, Dept Biol Chem, DNA Sequencing Core, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Dept Pharmacol, Ann Arbor, MI 48109 USA

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2010年 / 32卷 / 04期

关键词：

head and neck squamous carcinoma; human; unique cell lines; genotyping; tetranucleotide repeats; UM-SCC; IN-SITU HYBRIDIZATION; CROSS-CONTAMINATION; CHROMOSOME LOSS; CANCER; TUMOR; DNA; INTEGRIN; ANTIBODY; ANTIGEN; GENE;

D O I：

10.1002/hed.21198

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Background. We established multiple University of Michigan Squamous Cell Carcinoma (UM-SCC) cell lines. With time, these have been distributed to other labs all over the world. Recent scientific discussions have noted the need to confirm the origin and identity of cell lines in grant proposals and journal articles. We genotyped the UM-SCC cell lines in our collection to confirm their unique identity. Method. Early-passage UM-SCC cell lines were genotyped and photographed. Results. Thus far, 73 unique head and neck UM-SCC cell lines (from 65 donors, including 21 lines from 17 females) were genotyped. In 7 cases, separate cell lines were established from the same donor. Conclusions. These results will be posted on the UM Head and Neck SPORE Tissue Core website for other investigators to confirm that the UM-SCC cells used in their laboratories have the correct features. Publications using UM-SCC cell lines should confirm the genotype. (C) 2009 Wiley Periodicals, Inc. Head Neck 32: 417-426, 2010

引用

页码：417 / 426

页数：10

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