The SH3-fold family: Experimental evidence and prediction of variations in the folding pathways

被引:133
作者
Guerois, R [1 ]
Serrano, L [1 ]
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
SH3; domain; Sso7d; folding kinetics; folding prediction; transistion state;
D O I
10.1006/jmbi.2000.4234
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate the relationships between protein topology, amino acid sequence and folding mechanisms, the folding transition state of the Sso7d protein has been characterised both experimentally and theoretically. Although Sso7d protein has a similar topology to that of the SH3 domains, the structure of its transition state is different from that of alpha -spectrin and src SH3 domains previously studied. The folding algorithm, Fold-X, including an energy function with specific sequence features, accounts for these differences and reproduces with a good agreement the set of experimental phi double dagger (-U) values obtained for the three proteins. Our analysis shows that taking into account sequence features underlying protein topology is critical for an accurate prediction of the folding process. (C) 2000 Academic Press.
引用
收藏
页码:967 / 982
页数:16
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