Vasopressin V1a receptor signaling in a rat choroid plexus cell line

被引：2

作者：

Battle, T

Preisser, L

Marteau, V

Meduri, G

Lambert, M

Nitschke, R

Brown, PD

Corman, B ^{[1
]}

机构：

[1] CEA Saclay, Serv Biol Cellulaire, F-91191 Gif Sur Yvette, France

[2] CAMR, Salisbury, Wilts, England

[3] Hop Bichat, INSERM, U460, F-75877 Paris, France

[4] Univ Freiburg, Inst Physiol, D-7800 Freiburg, Germany

[5] Univ Manchester, Sch Biol Sci, Manchester, Lancs, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 275卷 / 02期

关键词：

choroid plexus; vasopressin; V1a receptor; cell line; desensitization; intracellular calcium;

D O I：

10.1006/bbrc.2000.3301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new cell line was derived from primary culture of rat choroid plexus (RCP) by immortalization with the TSOri minus adenovirus. The selected clone expressed vasopressin Via receptors at a density of 64,000 sites per cell, and a K-d of 7.2 nM. Addition of vasopressin to the RCP cells induced a transient calcium peak comparable to Via receptor signalling in different expression systems. This [Ca2+](i) increase was dose-dependent with an EC50 of 22 nM vasopressin. Similar [Ca2+](i) increase was elicited by addition of serotonin, angiotensin II, endothelin-1, and bradykinin, Heterologous desensitization of Via receptor was observed in RCP cells exposed to the phorbol ester PMA or following stimulation of other receptors coupled to the phosphoinositide pathway. Positive immunolabelling with Factor VIII, Flt1 and CD 34 antibodies suggests that this new RCP cell line originated from endothelial cells of rat choroid plexus. (C) 2000 Academic Press.

引用

页码：322 / 327

页数：6

共 33 条

[1] Serotonin, bradykinin and endothelin signalling in a sheep choroid plexus cell line [J].