A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone

被引：60

作者：

Leroy, E ^{[1
]}

Bensel, N ^{[1
]}

Reymond, JL ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 13期

关键词：

ENZYME FINGERPRINTS; FLUOROGENIC ASSAY; IN-VITRO; EVOLUTION;

D O I：

10.1016/S0960-894X(03)00377-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. lsobutyryloxymethyl ether le and pivaloxymethyl ether Id were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2105 / 2108

页数：4

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