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Dopamine D3 receptor antisense reduces neuropeptide mRNA levels in rat nucleus accumbens

被引：23
作者：
Tremblay, M
Rouillard, C
Lévesque, D
机构：
[1] CHUQ Pavillon CHUL, Ctr Rech, Unite Neurosci, Quebec City, PQ G1V 4G2, Canada
[2] Univ Laval, Fac Med, Dept Med, Div Pharmacol, Quebec City, PQ G1K 7P4, Canada
来源：
NEUROREPORT | 1997年 / 8卷 / 18期
关键词：
antisense oligonucleotide; dopamine D-3 receptor; dynorphin; in situ hybridization; neuroleptic; neurotensin; nucleus accumbens core; nucleus accumbens shell; radioreceptor assay; rat;
D O I：
10.1097/00001756-199712220-00012
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
WE have investigated the effects of a dopamine D-3 receptor antisense oligodeoxynucleotide (ODN), on neurotensin and dynorphin mRNA levels in rat nucleus accumbens. Intracerebroventricular injections of ODNs were made into the lateral ventricle (5 and 10 mu g h(-1), for 5 days). Receptor autoradiography of dopamine D-2 and D-3 receptor subtypes was performed. Dynorphin and neurotensin mRNA levels were evaluated by in situ hybridization. Dopamine D-3 receptor levels were significantly decreased in nucleus accumbens shell (NAS) of rats that received the D-3 antisense ODN. Dynorphin and neurotensin mRNA levels were also significantly decreased in the NAS after D-3 antisense ODN treatments. Our results show that D-3 receptors may regulate neuropeptide gene expression and demonstrate that an antisense strategy could be useful to identify molecular targets under control of specific dopamine receptor subtypes.
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页码：3901 / 3905
页数：5
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