学术探索
学术期刊
新闻热点
数据分析
智能评审

Labeless immunosensor assay for fluoroquinolone antibiotics based upon an AC impedance protocol

被引：22
作者：
Garifallou, G.-Z.
Tsekenis, G.
Davis, F.
Higson, S. P. J. [1 ]
Millner, P. A.
Pinacho, D. G.
Sanchez-Baeza, F.
Marco, M.-P.
Gibson, T. D.
机构：
[1] Cranfield Univ, Cranfield Hlth, Silsoe MK45 4DT, Beds, England
[2] Univ Leeds, Leeds, W Yorkshire, England
[3] CSIC, Inst Chem & Environm Res, IIQAB, Barcelona, Spain
[4] T&D Technol, Wakefield, W Yorkshire, England
来源：
ANALYTICAL LETTERS | 2007年 / 40卷 / 07期
关键词：
fluoroquinolone; ciprofloxacin; AC impedance; immunosensor; polyaniline;
D O I：
10.1080/00032710701327070
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper describes the construction of a labeless immunosensor for the antibiotic ciprofloxacin and its interrogation using an AC impedance protocol. Commercial screen-printed carbon electrodes were used as the basis for the sensor. Polyaniline was electrodeposited onto the sensors and then utilized to immobilize a biotinylated antibody for ciprofloxacin using classical avidin-biotin interactions. Electrodes containing the antibodies were exposed to solutions of antigen and interrogated using an AC impedance protocol. The faradaic component of the impedance of the electrodes was found to increase with increasing concentration of antigen. Control samples containing a non-specific IgG antibody were also studied and calibration curves obtained by subtraction of the responses for specific and non-specific antibody-based sensors, thereby eliminating the effects of non-specific adsorption of antigen.
引用
收藏
页码：1412 / 1422
页数：11
相关论文
共 17 条
[1]   DETERMINATION OF P-CRESOL (AND OTHER PHENOLICS) USING A CONDUCTING POLYMER-BASED ELECTRO-IMMUNOLOGICAL SENSING SYSTEM [J].
BARNETT, D ;
LAING, DG ;
SKOPEC, S ;
SADIK, O ;
WALLACE, GG .
ANALYTICAL LETTERS, 1994, 27 (13) :2417-2429
[2]   Evaluating the vulnerability of surface waters to antibiotic contamination from varying wastewater treatment plant discharges [J].
Batt, Angela L. ;
Bruce, Ian B. ;
Aga, Diana S. .
ENVIRONMENTAL POLLUTION, 2006, 142 (02) :295-302
[3]   Direct electrochemical immunosensor for polychlorinated biphenyls [J].
Bender, S ;
Sadik, OA .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (06) :788-797
[4]   ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY [J].
CLARK, LC ;
LYONS, C .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1962, 102 (01) :29-&
[5]   Affinity biosensors based on electropolymerized films [J].
Cosnier, S .
ELECTROANALYSIS, 2005, 17 (19) :1701-1715
[6]   Recent advances in electrochemical enzyme immunoassays [J].
Díaz-González, M ;
González-García, MB ;
Costa-García, A .
ELECTROANALYSIS, 2005, 17 (21) :1901-1918
[7]   Fluoroquinolones in paediatrics: a risk for the patient or for the community? [J].
Gendrel, D ;
Chalumeau, M ;
Moulin, F ;
Raymond, J .
LANCET INFECTIOUS DISEASES, 2003, 3 (09) :537-546
[8]   Label-free and reversible immunosensor based upon an ac impedance interrogation protocol [J].
Grant, S ;
Davis, F ;
Law, KA ;
Barton, AC ;
Collyer, SD ;
Higson, SPJ ;
Gibson, TD .
ANALYTICA CHIMICA ACTA, 2005, 537 (1-2) :163-168
[9]   Labeless and reversible immunosensor assay based upon an electrochemical current-transient protocol [J].
Grant, S ;
Davis, F ;
Pritchard, JA ;
Law, KA ;
Higson, SPJ ;
Gibson, TD .
ANALYTICA CHIMICA ACTA, 2003, 495 (1-2) :21-32
[10]   Primary DNA damage but not mutagenicity correlates with ciprofloxacin concentrations in German hospital wastewaters [J].
Hartmann, A ;
Golet, EM ;
Gartiser, S ;
Alder, AC ;
Koller, T ;
Widmer, RM .
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1999, 36 (02) :115-119
12