Cloning and characterization of the human RNA polymerase I subunit hRPA40

被引：7

作者：

Dammann, R ^{[1
]}

Pfeifer, GP ^{[1
]}

机构：

[1] City Hope Natl Med Ctr, Beckman Res Inst, Dept Biol, Duarte, CA 91010 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1396卷 / 02期

关键词：

RNA polymerase II; gene cloning; transcription;

D O I：

10.1016/S0167-4781(97)00206-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cloning of the human RNA polymerase I 40 kDa subunit, and the comparison of its amino acid sequence to other related RNA polymerase subunits are described. The amino acid sequence of hRPA40 has high homology to the mouse RNA polymerase I 40 kDa subunit (93%), to two Arabidopsis thaliana subunits (47%), the yeast RPC40 subunit (46%) and the human RNA polymerase II hRPB33 subunit (40%). Southern blot analysis shows that this gene is single copy and Northern blot analysis indicates that the mRNA of 1.3 kb is expressed in different cell types. (C) 1998 Elsevier Science B.V.

引用

页码：153 / 157

页数：5