Host cell caveolae act as an entry-port for group A streptococci

被引:86
作者
Rohde, M [1 ]
Müller, E [1 ]
Chhatwal, GS [1 ]
Talay, SR [1 ]
机构
[1] GBF German Res Ctr Biotechnol, Dept Microbial Pathogen & Vaccine Res, D-38124 Braunschweig, Germany
关键词
D O I
10.1046/j.1462-5822.2003.00279.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
This study identified caveolae as an entry port for group A streptococci into epithelial and endothelial cells. Scanning electron microscopy as well as ultrathin sections of infected cells demonstrated accumulation of small omega-shaped cavities in the host cell membrane close to adherent streptococci. During invasion, invaginations were formed that subsequentely revealed intracellular compartments surrounding streptococci. Caveolin-1 was shown to be present in the membrane of invaginations and the compartment membranes. These compartments were devoid of any classic endosomal/lysosomal marker proteins and can thus be described as caveosomes. Disruption of caveolae with methyl-beta-cyclodextrin and filipin abolished host cell invasion. Importantly, streptococci inside caveosomes avoid fusion with lysosomes. Expressing of SfbI protein on the surface of the non-invasive S. gordonii resulted in identical morphological alterations on the host cell as for S. pyogenes . Incubation of HUVEC cells with purified recombinant sole SfbI protein also triggered accumulation of cavity-like structures and formation of membrane invaginations. Tagged to colloidal gold-particles, SfbI protein was shown to cluster following membrane contact. Thus, our results demonstrate that host cell caveolae initiate the invasion process of group A streptococci and that the streptococcal invasin SfbI is the triggering factor that activates the caveolae-mediated endocytic pathway.
引用
收藏
页码:323 / 342
页数:20
相关论文
共 60 条
[1]   The caveolae membrane system [J].
Anderson, RGW .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :199-225
[2]   Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic [J].
Baorto, DM ;
Gao, ZM ;
Malaviya, R ;
Dustin, ML ;
vanderMerwe, A ;
Lublin, DM ;
Abraham, SN .
NATURE, 1997, 389 (6651) :636-639
[3]  
Chapman HA, 1999, THROMB HAEMOSTASIS, V82, P291
[4]   Cell biology of caveolae and caveolin [J].
Couet, J ;
Belanger, MM ;
Roussel, E ;
Drolet, MC .
ADVANCED DRUG DELIVERY REVIEWS, 2001, 49 (03) :223-235
[5]   Streptococcus pyogenes serotype M1 encodes multiple pathways for entry into human epithelial cells [J].
Cue, D ;
Dombek, PE ;
Lam, H ;
Cleary, PP .
INFECTION AND IMMUNITY, 1998, 66 (10) :4593-4601
[6]   Group A Streptococcus tissue invasion by CD44-mediated cell signalling [J].
Cywes, C ;
Wessels, MR .
NATURE, 2001, 414 (6864) :648-652
[7]   High-frequency intracellular invasion of epithelial cells by serotype M1 group A streptococci: M1 protein-mediated invasion and cytoskeletal rearrangements [J].
Dombek, PE ;
Cue, D ;
Sedgewick, J ;
Lam, H ;
Ruschkowski, S ;
Finlay, BB ;
Cleary, PP .
MOLECULAR MICROBIOLOGY, 1999, 31 (03) :859-870
[8]   Essential role for cholesterol in entry of mycobacteria into macrophages [J].
Gatfield, J ;
Pieters, J .
SCIENCE, 2000, 288 (5471) :1647-1650
[9]   Transduction - Integrin signaling [J].
Giancotti, FG ;
Ruoslahti, E .
SCIENCE, 1999, 285 (5430) :1028-1032
[10]   INVASION OF CULTURED HUMAN-CELLS BY STREPTOCOCCUS-PYOGENES [J].
GRECO, R ;
DEMARTINO, L ;
DONNARUMMA, G ;
CONTE, MP ;
SEGANTI, L ;
VALENTI, P .
RESEARCH IN MICROBIOLOGY, 1995, 146 (07) :551-560