Host cell caveolae act as an entry-port for group A streptococci

被引:86
作者
Rohde, M [1 ]
Müller, E [1 ]
Chhatwal, GS [1 ]
Talay, SR [1 ]
机构
[1] GBF German Res Ctr Biotechnol, Dept Microbial Pathogen & Vaccine Res, D-38124 Braunschweig, Germany
关键词
D O I
10.1046/j.1462-5822.2003.00279.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
This study identified caveolae as an entry port for group A streptococci into epithelial and endothelial cells. Scanning electron microscopy as well as ultrathin sections of infected cells demonstrated accumulation of small omega-shaped cavities in the host cell membrane close to adherent streptococci. During invasion, invaginations were formed that subsequentely revealed intracellular compartments surrounding streptococci. Caveolin-1 was shown to be present in the membrane of invaginations and the compartment membranes. These compartments were devoid of any classic endosomal/lysosomal marker proteins and can thus be described as caveosomes. Disruption of caveolae with methyl-beta-cyclodextrin and filipin abolished host cell invasion. Importantly, streptococci inside caveosomes avoid fusion with lysosomes. Expressing of SfbI protein on the surface of the non-invasive S. gordonii resulted in identical morphological alterations on the host cell as for S. pyogenes . Incubation of HUVEC cells with purified recombinant sole SfbI protein also triggered accumulation of cavity-like structures and formation of membrane invaginations. Tagged to colloidal gold-particles, SfbI protein was shown to cluster following membrane contact. Thus, our results demonstrate that host cell caveolae initiate the invasion process of group A streptococci and that the streptococcal invasin SfbI is the triggering factor that activates the caveolae-mediated endocytic pathway.
引用
收藏
页码:323 / 342
页数:20
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