Transgene flow to hybrid rice and its male-sterile lines

被引:44
作者
Jia, Shirong [1 ]
Wang, Feng
Shi, Lei
Yuan, Qianhua
Liu, Wuge
Liao, Yilong
Li, Shuguang
Jin, Wujun
Peng, Huipu
机构
[1] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
[2] Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 51064, Peoples R China
[3] Hainan Univ, Haikou 570228, Peoples R China
[4] Hainan Res & Dev Base Hybid Rice & Transgen Crops, Sanya 572000, Peoples R China
关键词
transgene flow; Oryza sativa; hybrid rice; male-sterile lines;
D O I
10.1007/s11248-006-9037-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Gene flow from genetically modified (GM) crops to the same species or wild relatives is a major concern in risk assessment. Transgenic rice with insect and/or disease resistance, herbicide, salt and/or drought tolerance and improved quality has been successfully developed. However, data on rice gene flow from environmental risk assessment studies are currently insufficient for the large-scale commercialization of GM rice. We have provided data on the gene flow frequency at 17 distances between a GM japonica line containing the bar gene as a pollen donor and two indica hybrid rice varieties and four male-sterile (ms) lines. The GM line was planted in a 640m(2) in an isolated experimental plot (2.4 ha), which simulates actual conditions of rice production with pollen competition. Results showed that: (1) under parallel plantation at the 0-m zone, the transgene flow frequency to the ms lines ranged from 3.145 to 36.116% and was significantly higher than that to hybrid rice cultivars (0.037-0.045%). (2) Gene flow frequency decreased as the distance increased, with a sharp cutoff point at about 1-2 m; (3) The maximum distance of transgene flow was 30-40 m to rice cultivars and 40-150 m to ms lines. We believe that these data will be useful for the risk assessment and management of transgenic rice lines, especially in Asia where 90% of world's rice is produced and hybrid rice varieties are extensively used.
引用
收藏
页码:491 / 501
页数:11
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