Detection of genes for alkane and naphthalene catabolism in Rhodococcus sp strain 1BN

被引:51
作者
Andreoni, V [1 ]
Bernasconi, S
Colombo, M
van Beilen, JB
Cavalca, L
机构
[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, I-20133 Milan, Italy
[2] Univ Milan, Dipartimento Chim Organ & Ind, I-20133 Milan, Italy
[3] ETH Honggerberg, Inst Biotechnol, CH-8093 Zurich, Switzerland
关键词
D O I
10.1046/j.1462-2920.2000.00134.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rhodococcus sp. 1BN was isolated from a contaminated site and showed various biodegradative capabilities. Besides naphthalene, strain 1BN degraded medium- (C-6) and long-chain alkanes (C-16-C-28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. The nucleotide sequence of an alk polymerase chain reaction (PCR) fragment revealed a 59% nucleotide homology to the Pseudomonas oleovorans alkB gene. The nar fragments were highly homologous to genes coding for large and small subunits of cis-naphthalene 1,2-dioxygenase (narAa and narAb) and to cis-naphthalene dihydrodiol dehydrogenase (narB) from other rhodococci, The oxidation of indene to cis-(1S,2R)-1,2-dihydroxyindan by toluene-induced cells allows to hypothesize that strain 1BN also carries a toluene dioxygenase-like system.
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收藏
页码:572 / 577
页数:6
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