Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay

被引：53

作者：

Borgers, Heleen ^{[1
]}

Moens, Leen ^{[1
]}

Picard, Capucine ^{[2
,3
]}

Jeurissen, Axel ^{[1
]}

Raes, Marc ^{[4
]}

Sauer, Kate ^{[4
]}

Proesmans, Marijke ^{[4
]}

De Boeck, Kris ^{[4
]}

Casanova, Jean-Laurent ^{[2
,5
]}

Meyts, Isabelle ^{[4
]}

Bossuyt, Xavier ^{[1
]}

机构：

[1] Catholic Univ Louvain, Biomed Sci Grp, Dept Med Diagnost Sci, Expt Med Lab, B-3000 Louvain, Belgium

[2] INSERM, Lab Human Genet Infect Dis, Necker Branch, U550, Paris, France

[3] Univ Paris 05, Necker Med Sch, Paris, France

[4] Univ Hosp Leuven, Dept Pediat, Louvain, Belgium

[5] Rockefeller Univ, Rockefeller Branch, Lab Human Genet Infect Dis, New York, NY 10021 USA

来源：

CLINICAL IMMUNOLOGY | 2010年 / 134卷 / 02期

关键词：

Immune deficiency; Streptococcus pneumoniae; Specific anti-polysaccharide immune deficiency; RESPIRATORY-TRACT INFECTIONS; PYOGENIC BACTERIAL-INFECTIONS; STREPTOCOCCUS-PNEUMONIAE; RECURRENT INFECTIONS; ANTICAPSULAR ANTIBODIES; HEALTHY-CHILDREN; TITERS; RESPONSES; IMMUNITY; DISEASE;

D O I：

10.1016/j.clim.2009.10.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated a multiplexed bead-based assay (xMAP (R) Pneumococcal Immunity assay from Luminex) for the simultaneous determination of antibodies against 14 capsular polysaccharides. Post-vaccination (Pneumovax (R)) antibody concentrations were measured in 35 healthy children, 40 healthy adults, 99 consecutive patients with increased susceptibility to respiratory infection, and 24 patients with a deficient anti-polysaccharide antibody response. The serotype-specific tower 5th percentile (cutoff) value for the post-immunization antibody concentration was determined in healthy individuals. Eleven of 99 patients (11%) failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested, whereas only 3 of 75 controls (4%) failed to do so. All. patients with known anti - polysaccharide antibody deficiency failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested. The XMAP (R) pneumococcal immunity panel appears useful for identifying individuals with a tow response to the unconjugated pneumococcal vaccine. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：198 / 205

页数：8

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