Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation

被引:298
作者
Boles, Blaise R. [1 ]
Thoendel, Matthew [1 ]
Roth, Aleeza J. [1 ]
Horswill, Alexander R. [1 ]
机构
[1] Univ Iowa, Dept Microbiol, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USA
来源
PLOS ONE | 2010年 / 5卷 / 04期
基金
美国国家科学基金会;
关键词
EXTRACELLULAR DNA; STREPTOCOCCUS-PNEUMONIAE; METHICILLIN RESISTANCE; PROTEOLYTIC ACTIVITY; BINDING PROTEINS; EXPRESSION; RELEASE; SYSTEM; AUTOLYSIS; SUSCEPTIBILITY;
D O I
10.1371/journal.pone.0010146
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Staphylococcus aureus is a potent biofilm former on host tissue and medical implants, and biofilm growth is a critical virulence determinant for chronic infections. Recent studies suggest that many clinical isolates form polysaccharide-independent biofilms. However, a systematic screen for defective mutants has not been performed to identify factors important for biofilm formation in these strains. We created a library of 14,880 mariner transposon mutants in a S. aureus strain that generates a proteinaceous and extracellular DNA based biofilm matrix. The library was screened for biofilm defects and 31 transposon mutants conferred a reproducible phenotype. In the pool, 16 mutants overproduced extracellular proteases and the protease inhibitor alpha(2)-macroglobulin restored biofilm capacity to 13 of these mutants. The other 15 mutants in the pool displayed normal protease levels and had defects in genes involved in autolysis, osmoregulation, or uncharacterized membrane proteins. Two transposon mutants of interest in the GraRS two-component system and a putative inositol monophosphatase were confirmed in a flow cell biofilm model, genetically complemented, and further verified in a community-associated methicillin-resistant S. aureus (CA-MRSA) isolate. Collectively, our screen for biofilm defective mutants identified novel loci involved in S. aureus biofilm formation and underscored the importance of extracellular protease activity and autolysis in biofilm development.
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页数:9
相关论文
共 56 条
[1]   Staphylocloccus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing [J].
Bae, T ;
Banger, AK ;
Wallace, A ;
Glass, EM ;
Åslund, F ;
Schneewind, O ;
Missiakas, DM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) :12312-12317
[2]   Global gene expression in Staphylococcus aureus biofilms [J].
Beenken, KE ;
Dunman, PM ;
McAleese, F ;
Macapagal, D ;
Murphy, E ;
Projan, SJ ;
Blevins, JS ;
Smeltzer, MS .
JOURNAL OF BACTERIOLOGY, 2004, 186 (14) :4665-4684
[3]   Microarray-based analysis of the Staphylococcus aureus σB regulon [J].
Bischoff, M ;
Dunman, P ;
Kormanec, J ;
Macapagal, D ;
Murphy, E ;
Mounts, W ;
Berger-Bächi, B ;
Projan, S .
JOURNAL OF BACTERIOLOGY, 2004, 186 (13) :4085-4099
[4]  
BISWAS R, 2006, FEMS MICROBIOL LETT, V268, P259
[5]   agr-mediated dispersal of Staphylococcus aureus biofilms [J].
Boles, Blaise R. ;
Horswill, Alexander R. .
PLOS PATHOGENS, 2008, 4 (04)
[6]   Identification of Staphylococcus aureus proteins recognized by the antibody-mediated immune response to a biofilm infection [J].
Brady, Rebecca A. ;
Leid, Jeff G. ;
Camper, Anne K. ;
Costerton, J. William ;
Shirtliff, Mark E. .
INFECTION AND IMMUNITY, 2006, 74 (06) :3415-3426
[7]   A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms [J].
Conrady, Deborah G. ;
Brescia, Cristin C. ;
Horii, Katsunori ;
Weiss, Alison A. ;
Hassett, Daniel J. ;
Herr, Andrew B. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (49) :19456-19461
[8]   The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation [J].
Corrigan, Rebecca M. ;
Rigby, David ;
Handley, Pauline ;
Foster, Timothy J. .
MICROBIOLOGY-SGM, 2007, 153 :2435-2446
[9]   Biofilm theory can guide the treatment of device-related orthopaedic infections [J].
Costerton, JW .
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 2005, (437) :7-11
[10]   Bap, a Staphylococcus aureus surface protein involved in biofilm formation [J].
Cucarella, C ;
Solano, C ;
Valle, J ;
Amorena, B ;
Lasa, I ;
Penadés, JR .
JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2888-2896