Organization of highly repeated sequences in surface-spread pachytene chromosomes of rye

被引：4

作者：

Cuñado, N ^{[1
]}

Barrios, J ^{[1
]}

Santos, JL ^{[1
]}

机构：

[1] Univ Complutense, Fac Biol, Dept Genet, E-28040 Madrid, Spain

来源：

GENOME | 2000年 / 43卷 / 06期

关键词：

fluorescence in situ hybridization; meiosis; repetitive DNA; rye; synaptonemal complex;

D O I：

10.1139/gen-43-6-945

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescence in situ hybridization (FISH) has been applied to analyze the location and organization of five different highly repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin displays different types of organization. Telomeric sequences were seen tightly associated with the SC while other repetitive DNA sequences were found to form loops that are associated with SCs only at their bases. On the contrary, the FISH signal of a centromeric satellite had a granular appearance, reflecting that the hybridization occurs only with parts of the chromatin loops.

引用

页码：945 / 948

页数：4

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