Biomarkers of cellular reaction to pesticide exposure in a rural population

被引：36

作者：

Fernanda Simoniello, Maria ^{[2
]}

Carlotta Kleinsorge, Elisa ^{[2
]}

Alberto Scagnetti, Jorge ^{[2
]}

Mastandrea, Carlos ^{[2
]}

Alberto Grigolato, Raul ^{[2
]}

Monica Paonessa, Adriana ^{[2
]}

Ana Carballo, Marta ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Dept Bioquim Clin, INFIBIOC,CIGETOX Citogenet Humana & Genet Toxicol, Buenos Aires, DF, Argentina

[2] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Catedra Toxicol Farmacol & Bioquim Legal, Santa Fe, Argentina

来源：

BIOMARKERS | 2010年 / 15卷 / 01期

关键词：

Oxidative stress; genotoxicity; comet assay; pesticides; human biomonitoring; LIPID-PEROXIDATION; OXIDATIVE STRESS; DNA-DAMAGE; OCCUPATIONAL-EXPOSURE; ANTIOXIDANT ENZYMES; RAT ERYTHROCYTES; WORKERS; GENOTOXICITY; GLUTATHIONE; INDUCTION;

D O I：

10.3109/13547500903276378

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study we report data obtained from the evaluation of subjects occupationally exposed to pesticide mixtures from Santa Fe province, Argentina, using biomarkers for butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities, catalase (CAT), lipid peroxidation (by TBARS assay) and the Damage Index Comet Assay (DICA). Our results showed an AChE inhibition (25% and 15% in directly and indirectly groups, respectively) in relation to controls with no significant modifications in BChE. TBARS levels were higher (51%) in pesticide sprayers while CAT activity was reduced in both, applicators (61%) and non-applicators (43%). DICA was significantly increased in direct (83%) and indirect (98%) exposed groups, compared with controls. These results showed modifications in lipid peroxidation, antioxidant defence system, and DNA damage in lymphocytes of exposed workers. Further investigations are suggested in order to link our findings with adverse health effects observed in chronic pesticide toxicity, where oxidative damage plays a pathophysiological role.</.

引用

页码：52 / 60

页数：9

共 45 条

[21] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].