Green fluorescent protein as a novel marker and reporter system in Helicobacter sp.

被引：23

作者：

Josenhans, C ^{[1
]}

Friedrich, S ^{[1
]}

Suerbaum, S ^{[1
]}

机构：

[1] Ruhr Univ Bochum, D-44780 Bochum, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 161卷 / 02期

关键词：

Helicobacter pylori; green fluorescent protein; gfp; reporter gene; flagellin;

D O I：

10.1016/S0378-1097(98)00068-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to be able to study gene regulation in single, live Helicobacter pylori bacteria in vitro or in contact with host cells, we established the green fluorescent protein gene gfp from Aequorea victoria as a reporter gene for use with Helicobacter species. We describe here the construction of genomic transcriptional fusions of the promoterless gfp gene with the flaA and flaB promoters of H. pylori. We have also constructed a Mini-Tn3-km-gfp transposon to be used for shuttle transposon mutagenesis in H. pylori and H. mustelae. A marker strain with wild-type phenotype, carrying multiple plasmid-borne copies of gfp under the control of the H. pylori flaB promoter, was constructed for studies of bacterial distribution and transmission in animal models. (C) 1998 Federation of European Microbiological societies. Published by Elsevier Science B.V.

引用

页码：263 / 273

页数：11

共 22 条

[11] CLONING OF CAMPYLOBACTER-JEJUNI GENES REQUIRED FOR LEUCINE BIOSYNTHESIS, AND CONSTRUCTION OF LEU-NEGATIVE MUTANT OF C-JEJUNI BY SHUTTLE TRANSPOSON MUTAGENESIS [J].