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Interlaboratory comparison of results of susceptibility testing with caspofungin against Candida and Aspergillus species
被引:137
作者:
Odds, FC
[1
]
Motyl, M
Andrade, R
Bille, J
Cantón, E
Cuenca-Estrella, M
Davidson, A
Durussel, C
Ellis, D
Foraker, E
Fothergill, AW
Ghannoum, MA
Giacobbe, RA
Gobernado, M
Handke, R
Laverdière, M
Lee-Yang, W
Merz, WG
Ostrosky-Zeichner, L
Pemán, J
Perea, S
Perfect, JR
Pfaller, MA
Proia, L
Rex, JH
Rinaldi, MG
Rodriguez-Tudela, JL
Schell, WA
Shields, C
Sutton, DA
Verweij, PE
Warnock, DW
机构:
[1] Univ Aberdeen, Inst Med Sci, Aberdeen Fungal Grp, Aberdeen AB25 2ZD, Scotland
[2] Merck Res Labs, Whitehouse Stn, NJ USA
[3] Univ Texas, Houston Med Sch, Houston, TX 77030 USA
[4] Univ Lausanne Hosp, Clin Microbiol Lab, CH-1011 Lausanne, Switzerland
[5] Univ Hosp La Fe, Microbiol Serv, Valencia 46009, Spain
[6] Inst Salud Carlos III, Natl Ctr Microbiol, Majadahonda 28220, Spain
[7] Womens & Childrens Hosp, Mycol Unit, Adelaide, SA, Australia
[8] Christiana Care Hlth Syst, Infect Dis Lab, Wilmington, DE 19801 USA
[9] Univ Texas, Hlth Sci Ctr, Dept Pathol, Fungus Testing Lab, San Antonio, TX 78229 USA
[10] Case Western Reserve Univ, Ctr Med Mycol, Dept Dermatol, Cleveland, OH 44106 USA
[11] Hop Maison Neuve Rosemont, Dept Microbiol Infect Dis, Montreal, PQ H1T 2M4, Canada
[12] Natl Ctr Infect Dis, Mycot Dis Branch, Div Bacterial & Mycot Dis, Ctr Dis Control & Prevent, Atlanta, GA 30333 USA
[13] Johns Hopkins Med Inst, Baltimore, MD 21287 USA
[14] Duke Univ, Med Ctr, Durham, NC 27710 USA
[15] Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA 52242 USA
[16] Rush Presbyterian St Lukes Med Ctr, Dept Med, Infect Dis Sect, Chicago, IL 60612 USA
[17] Radboud Univ Nijmegen Med Ctr, NL-6500 HB Nijmegen, Netherlands
关键词:
D O I:
10.1128/JCM.42.8.3475-3482.2004
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Seventeen laboratories participated in a study of interlaboratory reproducibility with caspofungin microdilution susceptibility testing against panels comprising 30 isolates of Candida spp. and 20 isolates of Aspergillus spp. The laboratories used materials supplied from a single source to determine the influence of growth medium (RPMI 1640 with or without glucose additions and antibiotic medium 3 [AM3]), the same incubation times (24 h and 48 h), and the same end point definition (partial or complete inhibition of growth) for the MIC of caspofungin. All tests were run in duplicate, and end points were determined both spectrophotometrically and visually. The results from almost all of the laboratories for quality control and reference Candida and Aspergillus isolates tested with fluconazole and itraconazole matched the NCCLS published values. However, considerable interlaboratory variability was seen in the results of the caspofungin tests. For Candida spp. the most consistent MIC data were generated with visual "prominent growth reduction" (MIC2) end points measured at 24 h in RPMI 1640, where 73.3% of results for the 30 isolates tested fell within a mode one dilution range across all 17 laboratories. MIC2 at 24 h in RPMI 1640 or AM3 also gave the best interlaboratory separation of Candida isolates of known high and low susceptibility to caspofungin. Reproducibility of MIC data was problematic for caspofungin tests with Aspergillus spp. under all conditions, but the minimal effective concentration end point, defined as the lowest caspofungin concentration yielding conspicuously aberrant hyphal growth, gave excellent reproducibility for data from 14 of the 17 participating laboratories.
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页码:3475 / 3482
页数:8
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