Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method

被引：10

作者：

Sivamani, E ^{[1
]}

Shen, P ^{[1
]}

Opalka, N ^{[1
]}

Beachy, RN ^{[1
]}

Fauquet, CM ^{[1
]}

机构：

[1] SCRIPPS RES INST, DIV PLANT BIOL, ORSTOM, TSRI, ILTAB, LA JOLLA, CA 92037 USA

来源：

PLANT CELL REPORTS | 1996年 / 15卷 / 05期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems, we have developed a procedure, using indica variety TN1 as a model that generates highly homogenous populations of embryo-genic subcultured calli by selectively propagating a small number of regeneration-proficient calli derived from seeds. Thousands of embryogenic calli were produced from 50 seeds within 10 weeks. Ten to 20 independent RO transgenic lines were regenerated per 500 embryogenic calli bombarded. The convenience and reliability offered by this transformation system has made transformation of indica rice a routine procedure.

引用

页码：322 / 327

页数：6

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