Electrophoresis of cereal storage proteins

被引:47
作者
Bean, SR
Lookhart, GL [1 ]
机构
[1] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA
[2] USDA ARS, Grain Marketing & Prod Res Ctr, Manhattan, KS 66502 USA
关键词
cereals; reviews; proteins;
D O I
10.1016/S0021-9673(99)01270-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cereal proteins have been studied by a number of analytical techniques over the years. One of the major methodologies utilized by cereal chemists has been electroaphoresis. Starting with moving boundary electrophoresis and progressing to slab gels and high-performance capillary electrophoresis, innovative methods have been developed to provide high resolution separations of difficult to separate proteins. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), acid-PAGE, isoelectric focusing, free zone CE, and even high-resolution two-dimensional HPLC-HPCE methods have been developed to separate cereal proteins. This review focuses on electrophoretic methods for separating and characterizing cereal storage proteins. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:23 / 36
页数:14
相关论文
共 96 条
[22]   AN IMPROVED SDS-PAGE METHOD FOR THE ANALYSIS OF WHEAT ENDOSPERM STORAGE PROTEINS [J].
GRAYBOSCH, RA ;
MORRIS, R .
JOURNAL OF CEREAL SCIENCE, 1990, 11 (03) :201-212
[23]   2-STEP ONE-DIMENSIONAL SDS-PAGE ANALYSIS OF LMW SUBUNITS OF GLUTELIN .1. VARIATION AND GENETIC-CONTROL OF THE SUBUNITS IN HEXAPLOID WHEATS [J].
GUPTA, RB ;
SHEPHERD, KW .
THEORETICAL AND APPLIED GENETICS, 1990, 80 (01) :65-74
[24]   Capillary sodium dodecyl sulfate-gel electrophoresis of proteins [J].
Guttman, A .
ELECTROPHORESIS, 1996, 17 (08) :1333-1341
[25]  
HAMAKER BR, 1995, CEREAL CHEM, V72, P583
[26]   CAPILLARY ELECTROPHORESIS OF PROTEINS AND NUCLEIC-ACIDS IN GELS AND ENTANGLED POLYMER-SOLUTIONS [J].
HELLER, C .
JOURNAL OF CHROMATOGRAPHY A, 1995, 698 (1-2) :19-31
[27]   FAST, HIGH-RESOLUTION (CAPILLARY) ELECTROPHORESIS IN BUFFERS DESIGNED FOR HIGH-FIELD STRENGTHS [J].
HJERTEN, S ;
VALTCHEVA, L ;
ELENBRING, K ;
LIAO, JL .
ELECTROPHORESIS, 1995, 16 (04) :584-594
[28]  
HOU G, 1995, CEREAL CHEM, V72, P545
[29]  
Juliano B.O., 1995, IDENTIFICATION FOOD, P265
[30]  
JULIANO BO, 1991, CEREAL CHEM, V68, P317