CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth

被引:32
作者
Cho-Chung, YS [1 ]
Park, YG [1 ]
Nesterova, M [1 ]
Lee, YN [1 ]
Cho, YS [1 ]
机构
[1] NCI, Cellular Biochem Sect, Tumor Immunol & Biol Lab, NIH, Bethesda, MD 20892 USA
关键词
cAMP; transcription factor-decoy oligonucleotides; CRE; Ap-1; p53; tumor growth; gene expression;
D O I
10.1023/A:1007144618589
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.
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页码:29 / 34
页数:6
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