Quantitative profiling of histone post-translational modifications by stable isotope labeling

被引：18

作者：

Knapp, Amy R.

Ren, Chen

Su, Xiaodan

Lucas, David M.

Byrd, John C.

Freitas, Michael A.

Parthun, Mark R. ^{[1
]}

机构：

[1] Ohio State Univ, Dept Mol & Cellular Biochem, Columbus, OH 43210 USA

[2] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Internal Med, Columbus, OH 43210 USA

来源：

METHODS | 2007年 / 41卷 / 03期

关键词：

histone; histone modifications; chromatin; mass spectrometry; stable isotope labeling;

D O I：

10.1016/j.ymeth.2006.08.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for accurately quantitating changes in histone post-translational modifications are necessary for developing an understanding of how their dynamic nature influences nuclear events involving access to genomic DNA. This article describes methods for the use of in vivo stable isotope label incorporation for quantitating the levels of modification at specific residues in histone proteins. These methods are applicable to a wide variety of model systems and examples of their use in both mammalian cells and Saccharomyces cerevisiae are presented. (c) 2006 Elsevier Inc. All rights reserved.

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收藏

页码：312 / 319

页数：8

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