Group-specific structural features of the 5′-proximal sequences of coronavirus genomic RNAs

被引：79

作者：

Chen, Shih-Cheng ^{[1
]}

Olsthoorn, Rene C. L. ^{[1
]}

机构：

[1] Leiden Univ, Leiden Inst Chem, Dept Mol Genet, Gorlaeus Labs, NL-2333 CC Leiden, Netherlands

来源：

VIROLOGY | 2010年 / 401卷 / 01期

关键词：

Coronavirus; SARS-CoV; RNA secondary structure; Packaging signal; 5 ' Untranslated region; CIS-ACTING ELEMENT; RESPIRATORY-SYNDROME CORONAVIRUS; VIRAL ENVELOPE PROTEIN; MURINE CORONAVIRUS; STEM-LOOP; PACKAGING SIGNAL; 5'-UNTRANSLATED REGION; SARS-CORONAVIRUS; LEADER; IDENTIFICATION;

D O I：

10.1016/j.virol.2010.02.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Global predictions of the secondary structure of coronavirus (Coy) 5 ' untranslated regions and adjacent coding sequences revealed the presence of conserved structural elements. Stem loops (SL) 1, 2, 4, and 5 were predicted in all CoVs, while the core leader transcription-regulating sequence (L-TRS) forms SL3 in only some CoVs. SL5 in group I and II CoVs, with the exception of group ha CoVs, is characterized by the presence of a large sequence insertion capable of forming hairpins with the conserved 5'-UUYCGU-3' loop sequence. Structure probing confirmed the existence of these hairpins in the group I Human coronavirus-229E and the group II Severe acute respiratory syndrome coronavirus (SARS-CoV). In general, the pattern of the 5' cis-acting elements is highly related to the lineage of CoVs, including features of the conserved hairpins in SL5. The function of these conserved hairpins as a putative packaging signal is discussed. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：29 / 41

页数：13

共 54 条

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