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Expression and function of interleukin-11 and its receptor α in the human endometrium

被引：36
作者：
Karpovich, N
Chobotova, K
Carver, J
Heath, JK
Barlow, DH
Mardon, HJ
机构：
[1] Univ Oxford, John Radcliffe Hosp, Dept Obstet & Gynaecol, Womens Ctr, Oxford OX3 9DU, England
[2] Univ Birmingham, Canc Res Campaign, Growth Factor Grp, Sch Biosci, Birmingham B15 2TT, W Midlands, England
来源：
MOLECULAR HUMAN REPRODUCTION | 2003年 / 9卷 / 02期
基金：
英国惠康基金;
关键词：
endometrium; heparin-binding epidermal growth factor; interleukin-11; interleukin-11 receptor alpha; proliferation; LEUKEMIA INHIBITORY FACTOR; MENSTRUAL-CYCLE; IMPLANTATION WINDOW; MOLECULAR-CLONING; GROWTH-FACTOR; CYTOKINES; CELLS; CHAIN; GENE; LOCUS;
D O I：
10.1093/molehr/gag012
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interleukin-11 (IL-11) receptor alpha has an important function in decidualization of mouse endometrial stroma but the function of IL-11 and its receptor in the human endometrium remains unknown. The mRNA for IL-11 and its receptor alpha in human endometrial tissue samples were analysed by semi-quantitative RT-PCR and RNase protection assays respectively. The proteins were detected in frozen endometrial tissue samples by immunofluorescence. The effect of heparin-binding epidermal growth factor (HB-EGF) on secretion of IL-11 by cultured endometrial stromal cells was assessed by enzyme-linked immunosorbent assay. The proliferative potential of IL-11 in endometrial stromal cells was assessed by [H-3]thymidine uptake. IL-11 and its receptor alpha mRNAs and proteins were detected in the endometrium throughout the cycle. Distinct patterns of localization of the ligand and receptor were observed. HB-EGF induced IL-11 secretion by cultured stromal cells, and IL-11 induced [H-3]thymidine uptake by these cells. Our data suggest that IL-11-receptor interactions may perform different functions in the human endometrium at different stages of the cycle, and that secretion of IL-11 is modulated by local growth factors.
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收藏
页码：75 / 80
页数：6
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