Antibacterial and allelopathic activity of extract from Castanea sativa leaves

被引:165
作者
Basile, A
Sorbo, S
Giordano, S
Ricciardi, L
Ferrara, S
Montesano, D
Cobianchi, RC
Vuotto, ML
Ferrara, L
机构
[1] Univ Naples Federico II, Dipartimento Biol Vegetale, I-80139 Naples, Italy
[2] Seconda Univ Naples, Fac Med & Chirurg, Ist Patol Gen & Oncol, Naples, Italy
[3] Univ Naples Federico II, Dipartimento Chim Farmaceut, Naples, Italy
关键词
Castanea sativa; flavonoids; antibacterial activity; allelopathic activity;
D O I
10.1016/S0367-326X(00)00185-4
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 mu g/ml and MBC in the range of 256-512 mu g/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:S110 / S116
页数:7
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