Fission yeast living mitosis visualized by GFP-tagged gene products

被引：41

作者：

Tatebe, H ^{[1
]}

Goshima, G ^{[1
]}

Takeda, K ^{[1
]}

Nakagawa, T ^{[1
]}

Kinoshita, K ^{[1
]}

Yanagida, M ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Dept Gene Mechanism, CREST Res Project,Sakyo Ku, Kyoto 6068502, Japan

来源：

MICRON | 2001年 / 32卷 / 01期

关键词：

green fluorescent protein; anaphase; fission yeast; S; pombe; mitosis; kinetochore; spindle pole body; tubulin; cyclin; centromere;

D O I：

10.1016/S0968-4328(00)00023-8

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The fission yeast Schizosaccharomyces pombe has been used as a model organism to study cell cycle control and dynamic chromosome behavior during anaphase segregation as genetic and cytological approaches are easily amenable. To understand the role of gene products involved in these cellular events, it is important to determine intracellular localization of each gene product during the cell cycle. In this article, visualization in living cells of several gene products involved in cell cycle control and sister chromatid separation is described. The genes tagged with jellyfish green fluorescent protein (GFP) include sad1(+) (encoding a spindle pole body (SPB) protein), atb2(+) (alpha-tubulin), mis6(+) (a kinetochore protein), eat1(+) (a novel actin-like protein localized in the nucleus) and cdc13(+) (a mitotic cyclin). In addition, LacI which is bound to a DNA segment containing LacO repeat sequences integrated near the centromere (cen1) is visualized. These are useful to monitor cell cycle events in living cells. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：67 / 74

页数：8

共 23 条

[1] DISTINCT NUCLEAR AND SPINDLE POLE BODY POPULATIONS OF CYCLIN-CDC2 IN FISSION YEAST [J].